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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Mary Elizabeth Felker; Indah Waty Bong; Walker H Depuy; Lina Farida Jihadah;

    Measurement, Reporting, and Verification (MRV) systems are thought to be essential for effective carbon accounting and joint REDD+ carbon, conservation, and social development goals. Community participation in MRV (PMRV) has been shown to be both cost effective and accurate, as well as a method to potentially advance stakeholder empowerment and perceptions of legitimacy. Recognizing land tenure as a long-standing point of tension in REDD+ planning, we argue that its engagement also has a key role to play in developing a legitimate PMRV. Using household surveys, key informant interviews, and participatory mapping exercises, we present three 'lived' land tenure contexts in Indonesia to highlight their socially and ecologically situated natures and to consider the role of tenure pluralism in shaping PMRV. We then raise and interrogate three questions for incorporating lived land tenure contexts into a legitimate PMRV system: 1) Who holds the right to conduct PMRV activities?; 2) How are the impacts of PMRV differentially distributed within local communities?; and 3) What is the relationship between tenure security and motivation to participate in PMRV? We conclude with implementation lessons for REDD+ practitioners, including the benefits of collaborative practices, and point to critical areas for further research.

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    PLoS ONE
    Article . 2017
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    PLoS ONE
    Article . 2017 . Peer-reviewed
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    Article . 2017
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    Authors: Mark A. Collinson; Michael J. White; Carren Ginsburg; F. Xavier Gómez-Olivé; +2 Authors

    The 2011 South African national census shows a cohort of young adults comprising an increasing share of the population. This finding is borne out in longitudinal data from the Agincourt Health and Demographic Surveillance System (HDSS). This primarily descriptive paper uses the Agincourt HDSS to examine the migration, employment and unemployment patterns in young adults. The study reveals high levels of temporary labour migration linking rural areas to metropolitan areas and secondary urban places. The type of work conducted by young adults in the Agincourt population is predominantly unskilled labour for both sexes. However, there is some evidence of female employment increasing in more educated sectors. Across all working ages there is pronounced unemployment, but the main pressure is felt by the younger adult population. Education and skills development for both sexes should be strengthened to support the country’s efforts to vastly improve labour force participation amongst the youth.

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    African Population Studies
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    African Population Studies
    Article . 2016 . Peer-reviewed
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    Europe PubMed Central
    Other literature type . 2016
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      African Population Studies
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      African Population Studies
      Article . 2016 . Peer-reviewed
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      Europe PubMed Central
      Other literature type . 2016
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    Authors: Steen Magnussen; Joan E. Luther; Richard A. Fournier; Joanne C. White; +1 Authors

    Forest inventory data often provide the required base data to enable the large area mapping of biomass over a range of scales. However, spatially explicit estimates of above-ground biomass (AGB) over large areas may be limited by the spatial extent of the forest inventory relative to the area of interest (i.e., inventories not spatially exhaustive), or by the omission of inventory attributes required for biomass estimation. These spatial and attributional gaps in the forest inventory may result in an underestimation of large area AGB. The continuous nature and synoptic coverage of remotely sensed data have led to their increased application for AGB estimation over large areas, although the use of these data remains challenging in complex forest environments. In this paper, we present an approach to generating spatially explicit estimates of large area AGB by integrating AGB estimates from multiple data sources; 1. using a lookup table of conversion factors applied to a non-spatially exhaustive forest inventory dataset (R2 = 0.64; RMSE = 16.95 t/ha), 2. applying a lookup table to unique combinations of land cover and vegetation density outputs derived from remotely sensed data (R2 = 0.52; RMSE = 19.97 t/ha), and 3. hybrid mapping by augmenting forest inventory AGB estimates with remotely sensed AGB estimates where there are spatial or attributional gaps in the forest inventory data. Over our 714,852 ha study area in central Saskatchewan, Canada, the AGB estimate generated from the forest inventory was approximately 40 Mega tonnes (Mt); however, the inventory estimate represents only 51% of the total study area. The AGB estimate generated from the remotely sensed outputs that overlap those made from the forest inventory based approach differ by only 2 %; however in total, the remotely sensed estimate is 30 % greater (58 Mt) than the estimate generated from the forest inventory when the entire study area is accounted for. Finally, using the hybrid approach, whereby the remotely sensed inputs were used to fill spatial gaps in the forest inventory, the total AGB for the study area was estimated at 62 Mt. In the example presented, data integration facilitates comprehensive and spatially explicit estimation of AGB for the entire study area.

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    Other literature type . Article . 2008 . Peer-reviewed
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    Sensors
    Article . 2008
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      Other literature type . Article . 2008 . Peer-reviewed
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    Authors: Allen Blackman; Leonardo Corral; Eirivelthon Lima; Gregory P. Asner;

    We thank Robinson et al. (1) for their thoughtful comments on our article (2). We agree with their main point, which is that our findings about the link between titling and forest cover change are context- and time-specific, and do not necessarily generalize to other countries and other periods. However, it is important to make clear that our article (2): ( i ) does not claim to present generalizable findings, ( ii ) nonetheless significantly advances our understanding of the above-noted link, and ( iii ) includes the four caveats discussed in the Robinson et al. letter (1). Regarding the first point, our article (2) demonstrates that titling indigenous communities in the Peruvian Amazon in the early 2000s … [↵][1]1To whom correspondence should be addressed. Email: allenb{at}iadb.org. [1]: #xref-corresp-1-1

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    Europe PubMed Central
    Other literature type . 2017
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    Proceedings of the National Academy of Sciences
    Article . 2017 . Peer-reviewed
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      Proceedings of the National Academy of Sciences
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    Authors: Julian M Norghauer; Adam R Martin; Erin E Mycroft; Arlington James; +1 Authors

    Despite its appeal to explain plant invasions, the enemy release hypothesis (ERH) remains largely unexplored for tropical forest trees. Even scarcer are ERH studies conducted on the same host species at both the community and biogeographical scale, irrespective of the system or plant life form. In Cabrits National Park, Dominica, we observed patterns consistent with enemy release of two introduced, congeneric mahogany species, Swietenia macrophylla and S. mahagoni, planted almost 50 years ago. Swietenia populations at Cabrits have reproduced, with S. macrophylla juveniles established in and out of plantation areas at densities much higher than observed in its native range. Swietenia macrophylla juveniles also experienced significantly lower leaf-level herbivory (∼3.0%) than nine co-occurring species native to Dominica (8.4-21.8%), and far lower than conspecific herbivory observed in its native range (11%-43%, on average). These complimentary findings at multiple scales support ERH, and confirm that Swietenia has naturalized at Cabrits. However, Swietenia abundance was positively correlated with native plant diversity at the seedling stage, and only marginally negatively correlated with native plant abundance for stems ≥1-cm dbh. Taken together, these descriptive patterns point to relaxed enemy pressure from specialized enemies, specifically the defoliator Steniscadia poliophaea and the shoot-borer Hypsipyla grandella, as a leading explanation for the enhanced recruitment of Swietenia trees documented at Cabrits.

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    Article . 2011
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    PLoS ONE
    Article . 2011 . Peer-reviewed
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    Authors: Dominik P. Guensch; Janelle Yu; Gobinath Nadeshalingam; Kady Fischer; +2 Authors

    BACKGROUND Previous serological studies have shown controversial results whether defibrillation or cardioversion can cause myocardial injury. Cardiovascular Magnetic Resonance (CMR) can be used to detect myocardial edema, hyperemia and capillary leak as features of acute myocardial injury. The aim of this study was to assess for myocardial and skeletal muscle injury in swine following transthoracic shocks. METHODS Seventeen anaesthetized swine were examined, with 11 undergoing five synchronized transthoracic shocks (200J). Myocardial and skeletal muscle injury were assessed at baseline and up to 5h post-shock employing T1 mapping, T2 mapping, early and late gadolinium enhancement. Serologic markers (cFABP, TnI, CK, and CK-MB) and myocardial tissue were assessed by standard histology methods. RESULTS In myocardial regions within the shock path, T1 and T2 were significantly increased compared to remote myocardium in the same animals. The early gadolinium enhancement ratio between the left-ventricular myocardium and the right pectoral muscle was also increased compared to control animals. After the shocks cFABP and CK were significantly elevated. After shock application, the regions identified as abnormal by CMR showed significantly increased interstitial and myocardial cell areas in histological analysis. This increased cell area suggests significant cellular and interstitial edema. CONCLUSION Our pilot study data indicate that serial defibrillator shocks lead to acute skeletal muscle and myocardial injury. CMR is a useful tool to detect and localize myocardial and skeletal muscle injury early after transthoracic shocks in vivo. In the future the technique could potentially be used as an additional tool for quality control such as verifying insufficient local shock application in non-responders after cardioversion or to develop safer shock forms.

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    Authors: Pesut, Barbara; Hooper, Brenda; Jacobsen, Marnie; Nielsen, Barbara; +2 Authors

    Abstract Background Few services are available to support rural older adults living at home with advancing chronic illness. The objective of this project was to pilot a nurse-led navigation service to provide early palliative support for rural older adults and their families living at home with advancing chronic illness. Methods Twenty-five older adults and 11 family members living with advancing chronic illness received bi-weekly home visits by a nurse navigator over a 2-year period. Navigation services included symptom management, education, advance care planning, advocacy, mobilization of resources, and psychosocial support. The nurse navigator collected longitudinal data on older adult and family needs, and older adult quality of life and healthcare utilization. Results Satisfaction with the service was high. There was no attrition over the 2-year period except through death, and few cancelled visits, indicating a high degree of acceptability of the intervention. The navigator addressed complex, multi-faceted needs through connecting health, social, and informal community resources. Participants who indicated a preferred place of death were able to die in that preferred place ( n = 7). Emergency room use by participants was minimal and largely unpreventable by the nurse navigator. Longitudinal health-related quality of life scores for many participants were poor, lending further support to the need for more focused attention to this upstream palliative population. Conclusions Using a nurse navigator to facilitate early palliative care for rural older adults living with advanced chronic illness is a promising innovation for meeting the needs of this population. Further research is required to evaluate outcomes on a larger scale.

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    BMC Palliative Care
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    Article . 2017
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    BMC Palliative Care
    Article . 2017 . Peer-reviewed
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    Authors: Nicole, Fenwick; Peter, Danielson; Gilly, Griffin;

    The ‘Three Rs’ tenet (replacement, reduction, refinement) is a widely accepted cornerstone of Canadian and international policies on animal-based science. The Canadian Council on Animal Care (CCAC) initiated this web-based survey to obtain greater understanding of ‘principal investigators’ and ‘other researchers’ (i.e. graduate students, post-doctoral researchers etc.) views on the Three Rs, and to identify obstacles and opportunities for continued implementation of the Three Rs in Canada. Responses from 414 participants indicate that researchers currently do not view the goal of replacement as achievable. Researchers prefer to use enough animals to ensure quality data is obtained rather than using the minimum and potentially waste those animals if a problem occurs during the study. Many feel that they already reduce animal numbers as much as possible and have concerns that further reduction may compromise research. Most participants were ambivalent about re-use, but expressed concern that the practice could compromise experimental outcomes. In considering refinement, many researchers feel there are situations where animals should not receive pain relieving drugs because it may compromise scientific outcomes, although there was strong support for the Three Rs strategy of conducting animal welfare-related pilot studies, which were viewed as useful for both animal welfare and experimental design. Participants were not opposed to being offered “assistance” to implement the Three Rs, so long as the input is provided in a collegial manner, and from individuals who are perceived as experts. It may be useful for animal use policymakers to consider what steps are needed to make replacement a more feasible goal. In addition, initiatives that offer researchers greater practical and logistical support with Three Rs implementation may be useful. Encouragement and financial support for Three Rs initiatives may result in valuable contributions to Three Rs knowledge and improve welfare for animals used in science.

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    Authors: Fleet, Richard; Archambault, Patrick; Légaré, France; Chauny, Jean-Marc; +8 Authors

    Introduction Emergency departments are important safety nets for people who live in rural areas. Moreover, a serious problem in access to healthcare services has emerged in these regions. The challenges of providing access to quality rural emergency care include recruitment and retention issues, lack of advanced imagery technology, lack of specialist support and the heavy reliance on ambulance transport over great distances. The Quebec Ministry of Health and Social Services published a new version of the Emergency Department Management Guide, a document designed to improve the emergency department management and to humanise emergency department care and services. In particular, the Guide recommends solutions to problems that plague rural emergency departments. Unfortunately, no studies have evaluated the implementation of the proposed recommendations. Methods and analysis To develop a comprehensive portrait of all rural emergency departments in Quebec, data will be gathered from databases at the Quebec Ministry of Health and Social Services, the Quebec Trauma Registry and from emergency departments and ambulance services managers. Statistics Canada data will be used to describe populations and rural regions. To evaluate the use of the 2006 Emergency Department Management Guide and the implementation of its various recommendations, an online survey and a phone interview will be administered to emergency department managers. Two online surveys will evaluate quality of work life among physicians and nurses working at rural emergency departments. Quality-of-care indicators will be collected from databases and patient medical files. Data will be analysed using statistical (descriptive and inferential) procedures. Ethics and dissemination This protocol has been approved by the CSSS Alphonse–Desjardins research ethics committee (Project MP-HDL-1213-011). The results will be published in peer-reviewed scientific journals and presented at one or more scientific conferences.

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    Europe PubMed Central
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    Authors: Renad Aljohani; Himeshi Samarasinghe; Tabi Ashu; Jianping Xu;

    AbstractYeasts are unicellular fungi; they are found in a diverse range of natural habitats, including soil, aquatic environments, the surface of plants, and the skin and mucosal surfaces of animal hosts. A variety of yeasts have been found in the soil environment. However, most studies of soil yeasts have come from developed countries, and there is a dearth of research on soil yeasts in Africa. In this study, we analyzed 493 soil samples from nine geographical locations in Cameroon for yeasts, using a culture - based method. A total of 110 yeast isolates were obtained. Based on their sequences at the fungal barcode locus, the Internal Transcribed Spacer (ITS) regions of the nuclear ribosomal RNA gene cluster, the 110 yeast isolates were putatively identified as belonging to 16 yeast species, including 15 Ascomycetes and one Basidiomycete. Differences in yeast species distribution were observed among the analyzed geographic regions. PCR fingerprinting analyses identified a large number of genotypes among strains within each of the obtained yeast species. Significantly, there was little evidence of geographic clustering among yeast strains from any of the yeast species. Our results suggest that Cameroon contains significant yeast diversity and that gene flow is common among local and regional soil yeast populations.

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    Scientific Reports
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    Authors: Mary Elizabeth Felker; Indah Waty Bong; Walker H Depuy; Lina Farida Jihadah;

    Measurement, Reporting, and Verification (MRV) systems are thought to be essential for effective carbon accounting and joint REDD+ carbon, conservation, and social development goals. Community participation in MRV (PMRV) has been shown to be both cost effective and accurate, as well as a method to potentially advance stakeholder empowerment and perceptions of legitimacy. Recognizing land tenure as a long-standing point of tension in REDD+ planning, we argue that its engagement also has a key role to play in developing a legitimate PMRV. Using household surveys, key informant interviews, and participatory mapping exercises, we present three 'lived' land tenure contexts in Indonesia to highlight their socially and ecologically situated natures and to consider the role of tenure pluralism in shaping PMRV. We then raise and interrogate three questions for incorporating lived land tenure contexts into a legitimate PMRV system: 1) Who holds the right to conduct PMRV activities?; 2) How are the impacts of PMRV differentially distributed within local communities?; and 3) What is the relationship between tenure security and motivation to participate in PMRV? We conclude with implementation lessons for REDD+ practitioners, including the benefits of collaborative practices, and point to critical areas for further research.

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    Authors: Mark A. Collinson; Michael J. White; Carren Ginsburg; F. Xavier Gómez-Olivé; +2 Authors

    The 2011 South African national census shows a cohort of young adults comprising an increasing share of the population. This finding is borne out in longitudinal data from the Agincourt Health and Demographic Surveillance System (HDSS). This primarily descriptive paper uses the Agincourt HDSS to examine the migration, employment and unemployment patterns in young adults. The study reveals high levels of temporary labour migration linking rural areas to metropolitan areas and secondary urban places. The type of work conducted by young adults in the Agincourt population is predominantly unskilled labour for both sexes. However, there is some evidence of female employment increasing in more educated sectors. Across all working ages there is pronounced unemployment, but the main pressure is felt by the younger adult population. Education and skills development for both sexes should be strengthened to support the country’s efforts to vastly improve labour force participation amongst the youth.

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    African Population Studies
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    African Population Studies
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      African Population Studies
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    Authors: Steen Magnussen; Joan E. Luther; Richard A. Fournier; Joanne C. White; +1 Authors

    Forest inventory data often provide the required base data to enable the large area mapping of biomass over a range of scales. However, spatially explicit estimates of above-ground biomass (AGB) over large areas may be limited by the spatial extent of the forest inventory relative to the area of interest (i.e., inventories not spatially exhaustive), or by the omission of inventory attributes required for biomass estimation. These spatial and attributional gaps in the forest inventory may result in an underestimation of large area AGB. The continuous nature and synoptic coverage of remotely sensed data have led to their increased application for AGB estimation over large areas, although the use of these data remains challenging in complex forest environments. In this paper, we present an approach to generating spatially explicit estimates of large area AGB by integrating AGB estimates from multiple data sources; 1. using a lookup table of conversion factors applied to a non-spatially exhaustive forest inventory dataset (R2 = 0.64; RMSE = 16.95 t/ha), 2. applying a lookup table to unique combinations of land cover and vegetation density outputs derived from remotely sensed data (R2 = 0.52; RMSE = 19.97 t/ha), and 3. hybrid mapping by augmenting forest inventory AGB estimates with remotely sensed AGB estimates where there are spatial or attributional gaps in the forest inventory data. Over our 714,852 ha study area in central Saskatchewan, Canada, the AGB estimate generated from the forest inventory was approximately 40 Mega tonnes (Mt); however, the inventory estimate represents only 51% of the total study area. The AGB estimate generated from the remotely sensed outputs that overlap those made from the forest inventory based approach differ by only 2 %; however in total, the remotely sensed estimate is 30 % greater (58 Mt) than the estimate generated from the forest inventory when the entire study area is accounted for. Finally, using the hybrid approach, whereby the remotely sensed inputs were used to fill spatial gaps in the forest inventory, the total AGB for the study area was estimated at 62 Mt. In the example presented, data integration facilitates comprehensive and spatially explicit estimation of AGB for the entire study area.

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    Authors: Allen Blackman; Leonardo Corral; Eirivelthon Lima; Gregory P. Asner;

    We thank Robinson et al. (1) for their thoughtful comments on our article (2). We agree with their main point, which is that our findings about the link between titling and forest cover change are context- and time-specific, and do not necessarily generalize to other countries and other periods. However, it is important to make clear that our article (2): ( i ) does not claim to present generalizable findings, ( ii ) nonetheless significantly advances our understanding of the above-noted link, and ( iii ) includes the four caveats discussed in the Robinson et al. letter (1). Regarding the first point, our article (2) demonstrates that titling indigenous communities in the Peruvian Amazon in the early 2000s … [↵][1]1To whom correspondence should be addressed. Email: allenb{at}iadb.org. [1]: #xref-corresp-1-1

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    Europe PubMed Central
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    Proceedings of the National Academy of Sciences
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    Authors: Julian M Norghauer; Adam R Martin; Erin E Mycroft; Arlington James; +1 Authors

    Despite its appeal to explain plant invasions, the enemy release hypothesis (ERH) remains largely unexplored for tropical forest trees. Even scarcer are ERH studies conducted on the same host species at both the community and biogeographical scale, irrespective of the system or plant life form. In Cabrits National Park, Dominica, we observed patterns consistent with enemy release of two introduced, congeneric mahogany species, Swietenia macrophylla and S. mahagoni, planted almost 50 years ago. Swietenia populations at Cabrits have reproduced, with S. macrophylla juveniles established in and out of plantation areas at densities much higher than observed in its native range. Swietenia macrophylla juveniles also experienced significantly lower leaf-level herbivory (∼3.0%) than nine co-occurring species native to Dominica (8.4-21.8%), and far lower than conspecific herbivory observed in its native range (11%-43%, on average). These complimentary findings at multiple scales support ERH, and confirm that Swietenia has naturalized at Cabrits. However, Swietenia abundance was positively correlated with native plant diversity at the seedling stage, and only marginally negatively correlated with native plant abundance for stems ≥1-cm dbh. Taken together, these descriptive patterns point to relaxed enemy pressure from specialized enemies, specifically the defoliator Steniscadia poliophaea and the shoot-borer Hypsipyla grandella, as a leading explanation for the enhanced recruitment of Swietenia trees documented at Cabrits.

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    Authors: Dominik P. Guensch; Janelle Yu; Gobinath Nadeshalingam; Kady Fischer; +2 Authors

    BACKGROUND Previous serological studies have shown controversial results whether defibrillation or cardioversion can cause myocardial injury. Cardiovascular Magnetic Resonance (CMR) can be used to detect myocardial edema, hyperemia and capillary leak as features of acute myocardial injury. The aim of this study was to assess for myocardial and skeletal muscle injury in swine following transthoracic shocks. METHODS Seventeen anaesthetized swine were examined, with 11 undergoing five synchronized transthoracic shocks (200J). Myocardial and skeletal muscle injury were assessed at baseline and up to 5h post-shock employing T1 mapping, T2 mapping, early and late gadolinium enhancement. Serologic markers (cFABP, TnI, CK, and CK-MB) and myocardial tissue were assessed by standard histology methods. RESULTS In myocardial regions within the shock path, T1 and T2 were significantly increased compared to remote myocardium in the same animals. The early gadolinium enhancement ratio between the left-ventricular myocardium and the right pectoral muscle was also increased compared to control animals. After the shocks cFABP and CK were significantly elevated. After shock application, the regions identified as abnormal by CMR showed significantly increased interstitial and myocardial cell areas in histological analysis. This increased cell area suggests significant cellular and interstitial edema. CONCLUSION Our pilot study data indicate that serial defibrillator shocks lead to acute skeletal muscle and myocardial injury. CMR is a useful tool to detect and localize myocardial and skeletal muscle injury early after transthoracic shocks in vivo. In the future the technique could potentially be used as an additional tool for quality control such as verifying insufficient local shock application in non-responders after cardioversion or to develop safer shock forms.

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    PLoS ONE
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    Authors: Pesut, Barbara; Hooper, Brenda; Jacobsen, Marnie; Nielsen, Barbara; +2 Authors

    Abstract Background Few services are available to support rural older adults living at home with advancing chronic illness. The objective of this project was to pilot a nurse-led navigation service to provide early palliative support for rural older adults and their families living at home with advancing chronic illness. Methods Twenty-five older adults and 11 family members living with advancing chronic illness received bi-weekly home visits by a nurse navigator over a 2-year period. Navigation services included symptom management, education, advance care planning, advocacy, mobilization of resources, and psychosocial support. The nurse navigator collected longitudinal data on older adult and family needs, and older adult quality of life and healthcare utilization. Results Satisfaction with the service was high. There was no attrition over the 2-year period except through death, and few cancelled visits, indicating a high degree of acceptability of the intervention. The navigator addressed complex, multi-faceted needs through connecting health, social, and informal community resources. Participants who indicated a preferred place of death were able to die in that preferred place ( n = 7). Emergency room use by participants was minimal and largely unpreventable by the nurse navigator. Longitudinal health-related quality of life scores for many participants were poor, lending further support to the need for more focused attention to this upstream palliative population. Conclusions Using a nurse navigator to facilitate early palliative care for rural older adults living with advanced chronic illness is a promising innovation for meeting the needs of this population. Further research is required to evaluate outcomes on a larger scale.

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    BMC Palliative Care
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    BMC Palliative Care
    Article . 2017 . Peer-reviewed
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    Authors: Nicole, Fenwick; Peter, Danielson; Gilly, Griffin;

    The ‘Three Rs’ tenet (replacement, reduction, refinement) is a widely accepted cornerstone of Canadian and international policies on animal-based science. The Canadian Council on Animal Care (CCAC) initiated this web-based survey to obtain greater understanding of ‘principal investigators’ and ‘other researchers’ (i.e. graduate students, post-doctoral researchers etc.) views on the Three Rs, and to identify obstacles and opportunities for continued implementation of the Three Rs in Canada. Responses from 414 participants indicate that researchers currently do not view the goal of replacement as achievable. Researchers prefer to use enough animals to ensure quality data is obtained rather than using the minimum and potentially waste those animals if a problem occurs during the study. Many feel that they already reduce animal numbers as much as possible and have concerns that further reduction may compromise research. Most participants were ambivalent about re-use, but expressed concern that the practice could compromise experimental outcomes. In considering refinement, many researchers feel there are situations where animals should not receive pain relieving drugs because it may compromise scientific outcomes, although there was strong support for the Three Rs strategy of conducting animal welfare-related pilot studies, which were viewed as useful for both animal welfare and experimental design. Participants were not opposed to being offered “assistance” to implement the Three Rs, so long as the input is provided in a collegial manner, and from individuals who are perceived as experts. It may be useful for animal use policymakers to consider what steps are needed to make replacement a more feasible goal. In addition, initiatives that offer researchers greater practical and logistical support with Three Rs implementation may be useful. Encouragement and financial support for Three Rs initiatives may result in valuable contributions to Three Rs knowledge and improve welfare for animals used in science.

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    Authors: Fleet, Richard; Archambault, Patrick; Légaré, France; Chauny, Jean-Marc; +8 Authors

    Introduction Emergency departments are important safety nets for people who live in rural areas. Moreover, a serious problem in access to healthcare services has emerged in these regions. The challenges of providing access to quality rural emergency care include recruitment and retention issues, lack of advanced imagery technology, lack of specialist support and the heavy reliance on ambulance transport over great distances. The Quebec Ministry of Health and Social Services published a new version of the Emergency Department Management Guide, a document designed to improve the emergency department management and to humanise emergency department care and services. In particular, the Guide recommends solutions to problems that plague rural emergency departments. Unfortunately, no studies have evaluated the implementation of the proposed recommendations. Methods and analysis To develop a comprehensive portrait of all rural emergency departments in Quebec, data will be gathered from databases at the Quebec Ministry of Health and Social Services, the Quebec Trauma Registry and from emergency departments and ambulance services managers. Statistics Canada data will be used to describe populations and rural regions. To evaluate the use of the 2006 Emergency Department Management Guide and the implementation of its various recommendations, an online survey and a phone interview will be administered to emergency department managers. Two online surveys will evaluate quality of work life among physicians and nurses working at rural emergency departments. Quality-of-care indicators will be collected from databases and patient medical files. Data will be analysed using statistical (descriptive and inferential) procedures. Ethics and dissemination This protocol has been approved by the CSSS Alphonse–Desjardins research ethics committee (Project MP-HDL-1213-011). The results will be published in peer-reviewed scientific journals and presented at one or more scientific conferences.

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    Europe PubMed Central
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    Authors: Renad Aljohani; Himeshi Samarasinghe; Tabi Ashu; Jianping Xu;

    AbstractYeasts are unicellular fungi; they are found in a diverse range of natural habitats, including soil, aquatic environments, the surface of plants, and the skin and mucosal surfaces of animal hosts. A variety of yeasts have been found in the soil environment. However, most studies of soil yeasts have come from developed countries, and there is a dearth of research on soil yeasts in Africa. In this study, we analyzed 493 soil samples from nine geographical locations in Cameroon for yeasts, using a culture - based method. A total of 110 yeast isolates were obtained. Based on their sequences at the fungal barcode locus, the Internal Transcribed Spacer (ITS) regions of the nuclear ribosomal RNA gene cluster, the 110 yeast isolates were putatively identified as belonging to 16 yeast species, including 15 Ascomycetes and one Basidiomycete. Differences in yeast species distribution were observed among the analyzed geographic regions. PCR fingerprinting analyses identified a large number of genotypes among strains within each of the obtained yeast species. Significantly, there was little evidence of geographic clustering among yeast strains from any of the yeast species. Our results suggest that Cameroon contains significant yeast diversity and that gene flow is common among local and regional soil yeast populations.

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    Scientific Reports
    Article . 2018
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    Scientific Reports
    Article . 2018 . Peer-reviewed
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