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ABSTRACT: To investigate the development and dynamics of green manure research, we collected 802 and 4588 papers on green manure research from China national knowledge infrastructure (CNKI) and Web of Science (WoS) between 1992 and 2021, respectively, and conducted quantitative and visual analyses based on bibliometric methods on the annual number of publications, authors and institutions, keywords, and emergent words. The main research hotspots in the field of green manure are discussed and summarized based on the results of the visualization analysis and the important publications explored by the results of the visualization analysis. The results showed that: the overall number of publications on green manure research exhibits an increasing trend, and research in this field is receiving more and more attention; the United States is the core country in this research field, with a great influence; although, the number of Chinese publications is large, the intermediary centrality value is small and the level of scientific research needs to be improved; the research characteristics in the field of green manure research mainly focus on green manure cultivation technology, the effects on soil and crops, and the effects on pests and diseases. The study was conducted based on CiteSpace, a visualization tool of bibliometrics, with comprehensive literature information and intuitive results, providing important references for relevant researchers to understand the extent of research in this field, academic communication, and grasp research dynamics.

ABSTRACT: Inclusion of rural youth is of great importance to facilitate nutritional security and make the food chain more sustainable. A positive attitude is necessary for active participation in agricultural activities. This case study was an attempt to check the attitude of rural youth regarding sustainable farming and its contributing factors. The study was carried out in the Punjab province of Pakistan and a transverse research design was used for this quantitative study. An interview schedule was used for getting the face-to-face response of rural youth. To make the unknown population of rural youth in Punjab, the Fisher formula was used and a sample of 413 was selected from purposely selected three districts of Punjab. After recording data, Statistical Package for Social Sciences (SPSS) was used to analyze the data. Descriptive Statistics was used to explain the response of rural youth for the decision on the base of data. Only 53 young farmers said that they have never been part of the formal education system and maximum number of farmers was close to 24 years of age or more than that. Rural youth of Punjab is lacking in non-formal education for acquiring or maintaining their skills & interests. Unemployment and having limited farm income are the biggest challenges that rural youth is facing. Rural youth show a strong inclination towards opting for sustainable farming alongside the availability of a supportive network. Regarding to learning capacities rural youth is lacking related to knowledge, communication means, novel approaches and in promoting sustainable farming initiatives. To achieve the goal of sustainability in changing environment, rural youth must have access to continuous training, communication sources, membership, or association with organizations with novel ideas covering all the changes happening in the farming business. RESUMO: Para facilitar a segurança nutricional e tornar a cadeia alimentar mais sustentável, a inclusão da juventude rural é de grande importância. Uma atitude positiva é necessária para a participação ativa nas atividades agrícolas. Este estudo de caso foi uma tentativa de verificar a atitude da juventude rural em relação à agricultura sustentável e seus fatores contribuintes. O trabalho foi realizado na província de Punjab, no Paquistão, e um projeto de pesquisa transversal foi usado para este estudo quantitativo. Um roteiro de entrevista foi usado para obter a resposta face a face da juventude rural. Para determinar a população desconhecida de jovens rurais em Punjab, a fórmula de Fisher foi usada e uma amostra de 413 foi selecionada de três distritos selecionados de Punjab. Após o registro dos dados, foi utilizado o Statistical Package for the Social Sciences (SPSS) para análise dos dados. A Estatística Descritiva foi utilizada para explicar a resposta dos jovens rurais para a decisão sobre a base de dados. Apenas 53 jovens agricultores disseram que nunca fizeram parte do sistema de educação formal e o número máximo de agricultores foi próximo aos 24 anos de idade ou mais. A juventude rural de Punjab carece de educação não formal para adquirir ou manter suas habilidades e interesses. Atualmente, o desemprego e a renda agrícola limitada são os maiores desafios que a juventude rural enfrenta. A juventude rural mostra uma forte inclinação para optar pela agricultura sustentável, juntamente com a disponibilidade de uma rede de apoio. No que diz respeito às capacidades de aprendizagem, a juventude rural carece de conhecimentos relacionados, meios de comunicação, novas abordagens e, até certo ponto, participação em organizações que promovem iniciativas de agricultura sustentável. Para alcançar a meta de sustentabilidade no ambiente em mudança, a juventude rural deve ter acesso a treinamento contínuo, fontes de comunicação, associação ou associação com organizações com ideias inovadoras que cubram todas as mudanças que ocorrem no negócio agrícola.

ABSTRACT: Avian-derived IgY is thought to be the best therapy for scorpion bites concerning low-level side effects. The present study analyzed a hypothesis about the neutralization of scorpion venom Androcotonus australis through antibodies produced in the egg yolks of chickens. The venom used for inoculation was obtained from Androctonus australis (yellow fat-tailed scorpion) from southern Punjab, Pakistan. The lethal dose of LD50 against scorpion venom was calculated in chickens and mice. Safe doses were given to egg-laying chickens to produce IgY antibodies. The antivenom IgY antibodies were extracted from the egg yolks of immunized chicken using the polyethylene glycol (PEG) method. Moreover, IgY was confirmed through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and the Ouchterlony double immunodiffusion assay test. The antibody titers were evaluated by the enzyme-linked immunosorbent assay (ELISA). The neutralisation capacity of extracted anti-scorpion antibodies was tested on mice. The calculated LD50 of scorpion venom for chicken and mice was 4 mg/kg and 2.5 mg/kg, respectively. SDS-PAGE and Ouchterlony double immunodiffusion confirmed the presence of IgY against scorpion venom. The maximum titer value of specific IgY produced against scorpion venom was 3.5 ug/ml. A concentration of 220 ul/LD50 was effective to neutralize 1 mg of scorpion venom. It is suggested that IgY obtained from egg yolks is safe against targeted venom and can be used as an effective alternative to equine IgG antibodies against scorpion envenoming.

ABSTRACT: Present study aimed for detection, purification, quantification of Sind Krait (Bungarus sindanus) antivenom from chicken eggs and to determine extracted antivenom efficacy in mice. Hens’ three groups were immunized by sub-lethal doses of Sind Krait venom with adjuvant paraffin oil+lecithin. Booster doses were injected subcutaneously on pectorals muscles at multiple sites after every two weeks upto eight weeks. Antibodies-IgY produced against Sind Krait venom was purified form eggs’ yolk by precipitation method with PEG-6000. Purified antivenom-IgY protein contents were quantified by Nanodrop-photometer, purity accessed by SDS-PAGE, specificity checked by Ochterloneys method and titer estimated by indirect ELISA. Antivenom efficacy was assessed in albino mice. Purified antivnom-IgY exhibited single protein band 180-190 kDa on SDS-PAGE under non-reduced condition and two-bands 63 - 65 kDa and 22 - 25 kDa correspondingly under-reduced condition. Immunodiffusion exhibited sharp precipitation lines of immune-complex (venom and extracted-IgY). In all groups (G1, G2 and G3) antivenom level sharply increase from 3rd to 4th week and maintained thereafter. G2 and G3 presented high titer upto 1:2048 dilutions, while G1 showed upto 1:1024 dilutions, as tested by indirect ELISA. In neutralization assay ED50 dose of G2 and G3 obtained antivenom was 400.23 µg/mice for more than twofold LD50 dose of venom and 100% protection was at 508.84 µg/mice that completely neutralized highly lethal dose of venom. But G1 ED50 was 405.66 µg/mice and provides 100% protection at 554.21 µg/mice. Extracted antivenom, against Sind Krait venom were highly pure, and with high neutralization capacity were produced successfully from eggs yolk first time in Pakistan.

Abstract A one year feeding trial was conducted on carps i.e. Catla (Cattla cattla), Mrigal (Cirhinus mrigala) and Rohu (Labeo rohita) to find out appropriate level of fishmeal in diet and their effects on growth, survival and biomass in intensive polyculture. Three different inclusions of fishmeal were used in experimental diets (25%, 35% and 45%). Highest average daily growth was observed by 25% fish meal diet (2.18g, 2.19g and 2.34g for catla, rohu and mrigal respectively), whereas 35% fish meal based diet was next by showing 1.63g average daily growth for catla, 1.73g for rohu and 1.67g for mrigal. Mean values of monthly weight again and average daily growth showed significant differences among treatments. Growth was found to be higher in C. mrigala in case of 25% and 45% and L. rohita in case of 35% fish meal. Minimum FCR was obtained by 25% FM based diets as 3.53±0.41 followed by 45% (3.82±0.33) and 35% (4.05±0.45). The findings of this research trial determine the optimum dietary level of fishmeal and its effectiveness as an important ingredient in diets of Indian major carps. It is proved that a feed by the combination of animal and plant protein is much preferable by carps as compare to a feed with higher concentration of fish meal.

Abstract Among edible mushrooms, Pleurotus eryngii is unique due to its flavor, admirable medicinal and nutritional profiling. Pakistan is an agricultural country diverse in various crops. However, the residues of the horticultural and agronomic crops are wasted without utilization in the food chain. Hence, a study was performed to assess the performance of relatively low-cost, easily available crops residues i.e. cotton, rice, wheat, mustard and water chestnut for yield and nutrition enhancement of Pleurotus eryngii strains P9 (China) and P10 (PSU-USA). The results revealed that morphological attributes i.e. mycelium run, fruit development, yield and biological efficiency were significantly higher by using cotton waste as compared to other substrates. Regarding biochemical attributes i.e. total soluble solids (12.67 °Brix), phenolics (259.6 mg/100g), moisture (92.3%) and ascorbic acid contents (2.9 mg/100ml) were also significantly higher by using cotton waste. Whereas, acidity (0.30%), reducing sugar (7.67%), non-reducing (4.33%) and total sugars contents (12%) were found highest by using mustard straw. Nutrient analysis of substrates showed that nutrient levels were increased after harvesting of crop as compared to before harvesting levels. Overall results revealed that cotton waste and mustard straw are promising substrates for Pleurotus eryngii better growth and have potential in yield and nutrition enhancement. Moreover, P10 strain performed better as compared to P9.

Between now and the near future, the Internet of Things (IoT) will redesign the socio-ecological morphology of the human terrain. The IoT ecosystem deploys diverse sensor platforms connecting millions of heterogeneous objects through the Internet. Irrespective of sensor functionality, most sensors are low energy consumption devices and are designed to transmit sporadically or continuously. However, when we consider the millions of connected sensors powering various user applications, their energy efficiency (EE) becomes a critical issue. Therefore, the importance of EE in IoT technology, as well as the development of EE solutions for sustainable IoT technology, cannot be overemphasised. Propelled by this need, EE proposals are expected to address the EE issues in the IoT context. Consequently, many developments continue to emerge, and the need to highlight them to provide clear insights to researchers on eco-sustainable and green IoT technologies becomes a crucial task. To pursue a clear vision of green IoT, this study aims to present the current state-of-the art insights into energy saving practices and strategies on green IoT. The major contribution of this study includes reviews and discussions of substantial issues in the enabling of hardware green IoT, such as green machine to machine, green wireless sensor networks, green radio frequency identification, green microcontroller units, integrated circuits and processors. This review will contribute significantly towards the future implementation of green and eco-sustainable IoT.

Abstract Rodents can cause significant damage to wheat-groundnut crops in developing countries, as well as to stored produce and infrastructure, affecting food security and income of small-holder farmers. Wheat (Triticum aestivum) and groundnuts (Arachis hypogea) are important cash crops for local farmers in Pakistan. Field experiments were performed to assess the extent of rodent damage to wheat-groundnut crops throughout their growth stages (i.e, germination, flowering/peg formation and maturity) in the agro-ecological zones of Pothwar Plateau, Pakistan. We used a quadrat method to record the number of damaged crop plants. On the basis of the trapping data four rodent species were captured from wheat-groundnut cropping systems which were responsible for causing damage, i.e., lesser bandicoot rat (Bandicota bengalensis) was the main species, followed by the short-tailed mole rat (Nesokia indica), the Indian gerbil (Tatera indica) and the bush rat (Golunda ellioti). In both crops, the maximum damage was recorded at crop maturity (10.7 and 14.4%, respectively). The lowest reported damage to wheat and groundnuts was at the germination stage (3.5% and 6.0%, respectively). The lower damage reported at germination could be due to availability of non-crop vegetation at field borders that may be a potential factor influencing damage. Our findings clearly show the considerable amount of damage caused by rodents to wheat-groundnut at maturity across all the agro-ecological zones of Pothwar and indicated that the small mammal composition was more related to maturity stage/season of crops, when the availability of food and climatic condition were favorable and having security under crop shelter. More detailed studies are needed to fully understand the population and breeding ecology of the relevant rodent pest species in relation to damage patterns to optimize management beyond individual structural measures.